integrin α6 apc  (R&D Systems)

Journal: PLoS ONE

Article Title: Highly Upregulated Lhx2 in the Foxn1 −/− Nude Mouse Phenotype Reflects a Dysregulated and Expanded Epidermal Stem Cell Niche

doi: 10.1371/journal.pone.0064223

Figure Lengend Snippet: CD49f/α6-integrin (blue) positive epithelium dominates within the basal layer of the surface epithelium. CD200 (purple) and CD34 (red) are both strongly expressed within the bulge region. In addition, CD200 is also expressed in upper parts of the outer root sheet (ORS) whereas CD34 expression may extend below the bulge region. Matrix cells of the hair bulb are considered CD49f/CD200/CD34 negative. In general, surface markers of epithelial progeny are not exclusive for each other in part depending on the stage of hair cycling. This study evaluates various effects of Foxn1 loss-of-function on telogen-anagen hair cycling and epithelial stem cell niche regulation in Nu/Nu mice.

Article Snippet: Epithelial isolates from newborn skin were labeled in 1x PBS buffer supplemented with 0.5% fetal calf serum (both www.invitrogen.com ) at 4°C for 40 min using following antibodies with isotype controls: APC-CD49f/α6-integrin (//us.ebioscience.com; #17-0495-80; isotype: #17-4321), PE-CD34 (// www.bdbiosciences.com ; #551387; isotype: #551799), PerCP-CD200 (//us.ebioscience.com; #46-5200-80; isotype: #45-4321 ).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Highly Upregulated Lhx2 in the Foxn1 −/− Nude Mouse Phenotype Reflects a Dysregulated and Expanded Epidermal Stem Cell Niche

doi: 10.1371/journal.pone.0064223

Figure Lengend Snippet: (A) Flow cytometry summary table listing fractions positive for markers of progeny within epithelial isolates from newborns (%±SD, n>6). Distinct differences between the Foxn1 −/− phenotype and wild type are demonstrated by comparative sample flow cytometry data gated for (B) CD49f, CD34, CD200 and (C) Oct3/4 positive epithelial fractions.

Article Snippet: Epithelial isolates from newborn skin were labeled in 1x PBS buffer supplemented with 0.5% fetal calf serum (both www.invitrogen.com ) at 4°C for 40 min using following antibodies with isotype controls: APC-CD49f/α6-integrin (//us.ebioscience.com; #17-0495-80; isotype: #17-4321), PE-CD34 (// www.bdbiosciences.com ; #551387; isotype: #551799), PerCP-CD200 (//us.ebioscience.com; #46-5200-80; isotype: #45-4321 ).

Techniques: Flow Cytometry

Journal: PLoS ONE

Article Title: Highly Upregulated Lhx2 in the Foxn1 −/− Nude Mouse Phenotype Reflects a Dysregulated and Expanded Epidermal Stem Cell Niche

doi: 10.1371/journal.pone.0064223

Figure Lengend Snippet: (A) Newborn epithelial isolates on day one post birth (NBD1) of Foxn1 −/− background show strong upregulation of Lhx2 which is not maintained following three days of in vitro culture along with other marker genes of progeny (ND = non-detectable). (B) However, CD200 levels remain higher in cultured Foxn1 −/− isolates compared to WT (qPCR/ΔΔCt-method: n = 4; *P<0.05, ↓P<0.05). (C) Flow cytometry on day 5 of in vitro cultures reveals a shift from a dominant CD49f++ subpopulation in vivo in favor of a CD34+/CD49f+ positive epithelial subpopulation in vivo which is more pronounced in Nu/Nu isolates vs. WT.

Article Snippet: Epithelial isolates from newborn skin were labeled in 1x PBS buffer supplemented with 0.5% fetal calf serum (both www.invitrogen.com ) at 4°C for 40 min using following antibodies with isotype controls: APC-CD49f/α6-integrin (//us.ebioscience.com; #17-0495-80; isotype: #17-4321), PE-CD34 (// www.bdbiosciences.com ; #551387; isotype: #551799), PerCP-CD200 (//us.ebioscience.com; #46-5200-80; isotype: #45-4321 ).

Techniques: In Vitro, Marker, Cell Culture, Flow Cytometry, In Vivo

Journal: Development (Cambridge, England)

Article Title: Diverse epithelial cell populations contribute to the regeneration of secretory units in injured salivary glands

doi: 10.1242/dev.192807

Figure Lengend Snippet: MECs do not contain highly proliferative progenitors. (A) FACS profile of Dox-induced K14-H2BGFP-labeled cells stained with APC-anti-integrin α6 antibody. Arrow indicates the ductal stem cell population expressing lower levels of integrin α6. (B) Cytospin analysis of sorted GFP+α6hi (MEC) and GFP+α6med (SC) cells. Scale bar: 20 μm. Graph shows percentages of K14+ (black bars) and SMA+ (gray bars) cells in each population (n=200 cells/population). (C) Colony formed from sorted cells seeded at 500 cell/well, grown for 2 weeks and stained with rhodamine. Scale bar: 1 mm. Graph shows colony-forming efficiency (diameter ≥0.5 mm). (D) Strategy used to induce label in either SMA+ or K14+ cells in vivo and analyze their fate in organoid cultures. (E) Images of organoids established from SMG cells of K14-TdT and SMA-TdT mice after 7 days in culture. Scale bar: 200 µm. (F) Percentage of TdT-labeled organoids in culture (>1100 organoids counted/gland). Values are mean±s.e.m, from at least two independent experiments. Two-tailed Student's t-test, P-values are indicated.

Article Snippet: For adherence cultures, single cell suspensions prepared from H2BGFP-labeled SMGs were re-suspended in PBS-1% bovine serum albumin and stained with APC-conjugated antibody to integrin α6 (clone GoH3, from eBioscience) for 20 min at 4°C in dark.

Techniques: Labeling, Staining, Expressing, In Vivo, Two Tailed Test

Journal: Scientific Reports

Article Title: Laminin-511-E8 promotes efficient in vitro expansion of human limbal melanocytes

doi: 10.1038/s41598-020-68120-0

Figure Lengend Snippet: List of antibodies used.

Article Snippet: CD49f (Integrin α6) APC (GoH3), rat , 1.25 μg/ml , Flow cytometry , eBioscience.

Techniques: Concentration Assay, Flow Cytometry, Blocking Assay, Immunohistochemistry, Immunocytochemistry